LOCUS       pLV-[hsa-miR-137]        9156 bp    DNA     circular     19-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7077..7937
                     /label=Amp(R)
     promoter        8138..8678
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5682..5916
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6491..6946)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5988..6118
                     /label=SV40 pA
     misc_feature    6119..6332
                     /label=SV40 ori
     misc_feature    5003..5599
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3621
                     /label=intron
     CDS             3668..4993
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3411
                     /label=hsa-mir-137 precursor
BASE COUNT     2149 a      2384 c      2512 g      2111 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg ggcgggctca 
     3121 gcgagcagca agagttctgg tggcggcggc ggcggcagta gcagcggcag cggtagcagc 
     3181 ggcagcggta gcagcggcag cggcagcttg gtcctctgac tctcttcggt gacgggtatt 
     3241 cttgggtgga taatacggat tacgttgtta ttgcttaaga atacgcgtag tcgaggagag 
     3301 taccagcggc aggggggcag cggccgccct ccccagccca ccagctggcc actaaacgcc 
     3361 cgtggttgcc aaggtagcac tttcttgttc ttttcatttc ctcgggtgtt tgctagctcg 
     3421 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc 
     3481 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg 
     3541 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa 
     3601 agtttttttc ttccatttca ggtgtcgtga aaactacccc tctagagtcg agctaccggt 
     3661 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg 
     3721 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg 
     3781 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc 
     3841 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt 
     3901 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg 
     3961 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct 
     4021 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg 
     4081 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga 
     4141 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta 
     4201 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta 
     4261 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca 
     4321 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaaggaccc 
     4381 accggtcgcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 
     4441 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 
     4501 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 
     4561 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 
     4621 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 
     4681 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 
     4741 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 
     4801 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 
     4861 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 
     4921 caccgtcacc gccgacgtcg agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa 
     4981 gcccggtgcc tgagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga 
     5041 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 
     5101 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 
     5161 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 
     5221 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 
     5281 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 
     5341 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 
     5401 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 
     5461 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 
     5521 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 
     5581 tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg 
     5641 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 
     5701 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 
     5761 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 
     5821 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 
     5881 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 
     5941 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 
     6001 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     6061 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 
     6121 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     6181 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     6241 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat 
     6301 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac 
     6361 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 
     6421 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 
     6481 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     6541 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     6601 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     6661 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     6721 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     6781 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     6841 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     6901 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc 
     6961 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
     7021 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 
     7081 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 
     7141 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 
     7201 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 
     7261 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 
     7321 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 
     7381 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 
     7441 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 
     7501 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 
     7561 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 
     7621 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 
     7681 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 
     7741 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 
     7801 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 
     7861 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 
     7921 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 
     7981 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     8041 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     8101 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     8161 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     8221 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 
     8281 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     8341 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     8401 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     8461 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     8521 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     8581 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     8641 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     8701 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     8761 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     8821 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 
     8881 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 
     8941 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 
     9001 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 
     9061 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa 
     9121 ttaaccctca ctaaagggaa caaaagctgg agctgc 
//
